This Tuesday (April 9th), I finally went back to RPI to continue my research! JP introduced me to a new project that the lab is starting. Due to property concerns, I can't go into it in detail, but it has to do with a protein that binds to transferrin, which then attaches to a receptor on endothelial cells. This protein can then be moved through the cell into the brain via receptor mediated endocytosis. To produce this protein, we are going to use a cloning vector in E. Coli, which we will remove the protein from via osmotic shock. Each of the target proteins have a flag sequence attached. This flag will allow us to use an antibody that binds to the flag to purify the protein.
Our first project, explained earlier, is involved with opening a pathway
between endothelial cells to allow transport into the brain. This new project also focuses on transport into the brain, but it is involved with making a pathway
through endothelial cells.
Kailin, you seem to be working on an important project! The sophistication with which you discuss this technology, and the privacy of the research suggests that your internship has moved to a new level.
ReplyDeleteAs always, your diagrams are terrific. They are wonderful complements to your internship and writing, and demonstrate a deep understanding of your activities.
Keep up the fantastic effort!